|
KMID : 1161420110140111352
|
|
Journal of Medicinal Food
2011 Volume.14 No. 11 p.1352 ~ p.1362
|
|
|
Preventing Bone Loss and Weight Gain with Combinations of Vitamin D and Phytochemicals
|
|
Lai Ching-Yi
Yang Jeong-Yeh
Rayalam Srujana
Della-Fera Mary Anne
Ambati Suresh
Lewis Richard D.
Hamrick Mark W.
Hartzell Diane L.
Baile Clifton A.
|
|
|
Abstract
|
|
|
|
Vitamin D and certain natural compounds have been shown to regulate both lipid metabolism and bone formation. Treatments that prevent or reverse age-related increase in bone marrow adiposity could both increase new bone formation and inhibit bone destruction. We tested the hypothesis that dietary supplementation with combinations of vitamin D and phytochemicals inhibits bone loss and decreases adiposity to a greater extent than control or vitamin D?alone diets. Aged ovariectomized female rats (12 months old, n=50, initial body weight=240?g) were given control (AIN-93M diet), vitamin D (2,400?IU/kg), or vitamin D plus resveratrol (16, 80, or 400?mg/kg of diet [low, medium, and high dose, respectively]), quercetin (80, 400, or 2,000?mg/kg of diet), and genistein (64, 256, or 1,040?mg/kg of diet) for 8 weeks. The high-dose treatment (vitamin D+400?mg/kg resveratrol+2,000?mg/kg quercetin+1,040?mg/kg genistein) reduced body weight gain (P<.05) and the fat pad weights (P<.05). This treatment also increased the serum concentration of insulin-like growth factor-1 (P<.05) and the bone mineral content of the femur. Micro-computed tomography and histomorphometric analyses indicated that the high-dose treatment prevented loss of trabecular bone (P<.05) and reduced marrow adipocytes (P<.001) and osteoclasts (P<.05) compared with the control and vitamin D alone (P<.05). We conclude that aged ovariectomized female rats supplemented with vitamin D combined with genistein, quercetin, and resveratrol had improved bone mineral density and reduced body weight gain and a significant decrease in bone marrow adipocytes. The synergistic effects of a combination of phytochemicals with vitamin D may be effective in reducing bone loss and weight gain after menopause.
|
|
|
KEYWORD
|
|
|
bone loss, genistein, osteoporosis, ovariectomized rats, quercetin, resveratrol
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|